Carboxy-group modification: high-temperature activation of charge-neutralized and charge-reversed beta-glucosidases from Aspergillus niger.

@article{Rashid1998CarboxygroupMH,
  title={Carboxy-group modification: high-temperature activation of charge-neutralized and charge-reversed beta-glucosidases from Aspergillus niger.},
  author={Muhammad Hamid Rashid and Khawar Sohail Siddiqui},
  journal={Biotechnology and applied biochemistry},
  year={1998},
  volume={27 ( Pt 3)},
  pages={231-7}
}
Purified beta-glucosidase from Aspergillus niger NIAB280 was chemically modified by l-ethyl-3-(3-dimethyl-aminopropyl)-carbodi-imide (EDC) in the presence of glycinamide (GAM) as nucleophile under various conditions to study the role of carboxy groups in the catalytic mechanism of this enzyme. beta-Glucosidase was inactivated by the binding of one mol of EDC per mol of the enzyme with a second-order rate constant of 4.77 x 10(-2) mM min-1. Glucose, as competitive inhibitor, partly protected the… CONTINUE READING