Carbonylated proteins in aging and exercise: immunoblot approaches.

  title={Carbonylated proteins in aging and exercise: immunoblot approaches.},
  author={Sataro Goto and Atsushi Nakamura and Zsolt Rad{\'a}k and Hideko Nakamoto and Ryoya Takahashi and Kayo Yasuda and Yasuko Sakurai and Naoaki Ishii},
  journal={Mechanisms of ageing and development},
  volume={107 3},
Protein carbonyls were studied in aging and exercise by immunoblot followed by one- or two-dimensional polyacrylamide gel electrophoresis using antibodies against 2,4-dinitrophenylhydrazones. Proteins of rat kidneys exhibited significant age-related increase in the amount of carbonyl while those of the brain and liver did not. Major carbonylated proteins in… CONTINUE READING