Carbonic Anhydrase Activity of Integral-functional Complexes of Thylakoid Membranes of Spinach Chloroplasts.

@article{Semenihin2015CarbonicAA,
  title={Carbonic Anhydrase Activity of Integral-functional Complexes of Thylakoid Membranes of Spinach Chloroplasts.},
  author={A V Semenihin and O K Zolotareva},
  journal={Ukrainian biochemical journal},
  year={2015},
  volume={87 3},
  pages={47-56}
}
Isolated thylakoid membranes were disrupted by treatment with nonionic detergents digitonin or dodecyl maltoside. Solubilized polypeptide complexes were separated by native gel charge shift electrophoresis. The position of ATP-synthase complex and its isolated catalytic part (CF1) within gel was determined using the color reaction for ATPase activity. Due… CONTINUE READING