Carbon monoxide stimulates the apical 70-pS K+ channel of the rat thick ascending limb.

@article{Liu1999CarbonMS,
  title={Carbon monoxide stimulates the apical 70-pS K+ channel of the rat thick ascending limb.},
  author={Huajun Liu and David B. Mount and Alberto Nasjletti and Wenhui Wang},
  journal={The Journal of clinical investigation},
  year={1999},
  volume={103 7},
  pages={963-70}
}
We have investigated the expression of heme oxygenase (HO) in the rat kidney and the effects of HO-dependent heme metabolites on the apical 70-pS K+ channel in the thick ascending limb (TAL). Reverse transcriptase-PCR (RT-PCR) and Western blot analyses indicate expression of the constitutive HO form, HO-2, in the rat cortex and outer medulla. Patch-clamping showed that application of 10 microM chromium mesoporphyrin (CrMP), an inhibitor of HO, reversibly reduced the activity of the apical 70-pS… CONTINUE READING