Carbon monoxide disposition in the perfused rat liver.

  title={Carbon monoxide disposition in the perfused rat liver.},
  author={David G. Le Couteur and Zhan Li Yin and Laurent P. Rivory and A J Mclean},
  journal={American journal of physiology. Gastrointestinal and liver physiology},
  volume={277 3},
A simple method for determining carbon monoxide (CO) disposition in the rat liver perfused with erythrocyte-free buffer was developed. Wash-in experiments were performed with buffer containing tracer quantities of [14C]sucrose and3H2O and equilibrated with CO. Outflow samples were collected into tubes containing human erythrocytes, which avidly bind CO. Outflow curves were analyzed using compartmental models. Fractional recovery of CO was 1.07 ± 0.17, and the apparent volume of distribution was… CONTINUE READING