Carbon limitation induces sigma(S)-dependent gene expression in Pseudomonas fluorescens in soil.

  title={Carbon limitation induces sigma(S)-dependent gene expression in Pseudomonas fluorescens in soil.},
  author={Birgit Koch and Jakob Worm and Linda Elise Jensen and Ole H\ojberg and Ole Nybroe},
  journal={Applied and environmental microbiology},
  volume={67 8},
Recent studies employing reporter gene technology indicate that the availabilities of the major nutrients nitrogen, phosphate, and iron to Pseudomonas are not severely limited in bulk soil. Indirect evidence has pointed to carbon limitation as a severe nutritional stress in this environment. We show that a plasmid (pGM115)-borne transcriptional fusion between the sigma(S)-dependent Escherichia coli promoter P(fic) and lacZ functions as a reliable reporter for carbon availability in Pseudomonas… CONTINUE READING


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