Carbon limitation induces sigma(S)-dependent gene expression in Pseudomonas fluorescens in soil.

@article{Koch2001CarbonLI,
  title={Carbon limitation induces sigma(S)-dependent gene expression in Pseudomonas fluorescens in soil.},
  author={Birgit Koch and Jakob Worm and Linda Elise Jensen and Ole H\ojberg and Ole Nybroe},
  journal={Applied and environmental microbiology},
  year={2001},
  volume={67 8},
  pages={3363-70}
}
Recent studies employing reporter gene technology indicate that the availabilities of the major nutrients nitrogen, phosphate, and iron to Pseudomonas are not severely limited in bulk soil. Indirect evidence has pointed to carbon limitation as a severe nutritional stress in this environment. We show that a plasmid (pGM115)-borne transcriptional fusion between the sigma(S)-dependent Escherichia coli promoter P(fic) and lacZ functions as a reliable reporter for carbon availability in Pseudomonas… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 23 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 44 references

Experiments in molecular genetics

J. H. Miller
Cold Spring Harbor Laboratory, • 1972
View 12 Excerpts
Highly Influenced

Pseudomonas fluorescens Tn5–B20 mutant RA92 responds to carbon limitation in soil

L. S. Van Overbeek, J. D. Van Elsas, J. A. Van Veen
FEMS Microbiol • 1997
View 5 Excerpts
Highly Influenced

Expression of a nitrogen regulated lux gene fusion in Pseudomonas fluorescens DF57 studied in pure culture and in soil

L. E. Jensen, L. Kragelund, O. Nybroe
FEMS Microbiol • 1998
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…