Carbohydrate-specific adhesion of bacteria to thin-layer chromatograms: a rationalized approach to the study of host cell glycolipid receptors.

@article{Hansson1985CarbohydratespecificAO,
  title={Carbohydrate-specific adhesion of bacteria to thin-layer chromatograms: a rationalized approach to the study of host cell glycolipid receptors.},
  author={Gunnar C Hansson and Kim Alexsander Karlsson and G. Olof. Larson and Nicklas Str{\"o}mberg and Jan Thurin},
  journal={Analytical biochemistry},
  year={1985},
  volume={146 1},
  pages={
          158-63
        }
}
Conditions have been adapted for the binding of intact bacteria to glycosphingolipids in a thin-layer chromatogram. Bacteria labeled externally with 125I or metabolically with other isotopes are layered on the plate and after repeated washing the bound bacteria are detected by autoradiography. Using this technique several kinds of bacteria have been shown to adhere to the plate in a carbohydrate-specific way with practically no background binding. Among the advantages of the method is the… CONTINUE READING
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