Carbodiimide modification enhances activity of pig pancreatic phospholipase A2.

  title={Carbodiimide modification enhances activity of pig pancreatic phospholipase A2.},
  author={Jo{\~a}o Pinto Ferreira and Ram Sasisekharan and Omid Louie and Robert S. Langer},
  journal={European journal of biochemistry},
  volume={223 2},
Pig phospholipase A2, pig iso-phospholipase A2 and bovine pancreatic phospholipase A2 were reacted in solution with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, in the presence of N-hydroxysulfosuccinimide, at pH 7. The influence of micellar protectants was analyzed. In the presence of n-hexadecylphosphocholine, the losses of activity in micellar diheptanoyl-lecithin were 80, 35, and 10% in bovine phospholipase A2, pig iso-phospholipase A2, and pig phospholipase A2, respectively. With 1… 
6 Citations
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