Capturing the mutational landscape of the beta-lactamase TEM-1.

  title={Capturing the mutational landscape of the beta-lactamase TEM-1.},
  author={Herv{\'e} Jacquier and Andr{\'e} Birgy and Herv{\'e} Le Nagard and Yves Mechulam and Emmanuelle Schmitt and J{\'e}r{\'e}my Glodt and B{\'e}atrice Bercot and Emmanuelle Petit and Julie Poulain and Guil{\`e}ne Barnaud and Pierre-Alexis Gros and Olivier A. Tenaillon},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={110 32},
Adaptation proceeds through the selection of mutations. The distribution of mutant fitness effect and the forces shaping this distribution are therefore keys to predict the evolutionary fate of organisms and their constituents such as enzymes. Here, by producing and sequencing a comprehensive collection of 10,000 mutants, we explore the mutational landscape of one enzyme involved in the spread of antibiotic resistance, the beta-lactamase TEM-1. We measured mutation impact on the enzyme activity… CONTINUE READING
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