Capturing diversity of marine heterotrophic protists: one cell at a time

@article{Heywood2011CapturingDO,
  title={Capturing diversity of marine heterotrophic protists: one cell at a time},
  author={Jane L. Heywood and Michael E. Sieracki and Wendy K. Bellows and Nicole Jane Poulton and Ramunas Stepanauskas},
  journal={The ISME Journal},
  year={2011},
  volume={5},
  pages={674-684}
}
Recent applications of culture-independent, molecular methods have revealed unexpectedly high diversity in a variety of functional and phylogenetic groups of microorganisms in the ocean. However, none of the existing research tools are free from significant limitations, such as PCR and cloning biases, low phylogenetic resolution and others. Here, we employed novel, single-cell sequencing techniques to assess the composition of small (<10 μm diameter), heterotrophic protists from the Gulf of… CONTINUE READING
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