Captured metagenomics: large-scale targeting of genes based on ‘sequence capture’ reveals functional diversity in soils

@inproceedings{Manoharan2015CapturedML,
  title={Captured metagenomics: large-scale targeting of genes based on ‘sequence capture’ reveals functional diversity in soils},
  author={Lokeshwaran Manoharan and Sandeep K. Kushwaha and Katarina Hedlund and Dag G. Ahr{\'e}n},
  booktitle={DNA research : an international journal for rapid publication of reports on genes and genomes},
  year={2015}
}
Microbial enzyme diversity is a key to understand many ecosystem processes. Whole metagenome sequencing (WMG) obtains information on functional genes, but it is costly and inefficient due to large amount of sequencing that is required. In this study, we have applied a captured metagenomics technique for functional genes in soil microorganisms, as an alternative to WMG. Large-scale targeting of functional genes, coding for enzymes related to organic matter degradation, was applied to two… CONTINUE READING
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A high frequency of activating extracellular domain ERBB2 (HER2) mutation in micropapillary urothelial carcinoma.

  • Clinical cancer research : an official journal of the American Association for Cancer Research
  • 2014

Tackling soil diversity with the assembly of large, complex metagenomes.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 2014
VIEW 1 EXCERPT