Capture immunoassay for the diagnosis of bovine mastitis using a monoclonal antibody to polymorphonuclear granulocytes.

@article{OSullivan1992CaptureIF,
  title={Capture immunoassay for the diagnosis of bovine mastitis using a monoclonal antibody to polymorphonuclear granulocytes.},
  author={Christine A O'Sullivan and Peter J. Joyce and T P Sloan and A G Shattock},
  journal={The Journal of dairy research},
  year={1992},
  volume={59 2},
  pages={123-33}
}
A direct capture enzyme-linked immunosorbent assay (ELISA) was developed to measure elevated polymorphonuclear granulocyte (PMN) antigens using horseradish peroxidase (EC 1.11.1.7) conjugated rabbit polyclonal anti-PMN antisera and a monoclonal antibody specific for PMN cells. Optical densities obtained in the ELISA were used to predict the cell counts of… CONTINUE READING