Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates

@inproceedings{Menezes2003CaptureIF,
  title={Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates},
  author={Caroline Anunciaç{\~a}o Menezes and Danielle Silva Gonçalves and Jackeline Amianti and Irene Fernandes and Carla Romano Taddei and Paula C{\'e}lia Mariko Koga and Luiz Rachid Trabulsi and Marina Baquerizo Martinez and Roxane M. F. Piazza},
  year={2003}
}
A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has been developed. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture… CONTINUE READING

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