Capture and visualization of live Mycobacterium tuberculosis bacilli from tuberculosis bioaerosols

@article{Dinkele2019CaptureAV,
  title={Capture and visualization of live Mycobacterium tuberculosis bacilli from tuberculosis bioaerosols},
  author={Ryan Dinkele and Sophia Gessner and A. Koch and Carl Morrow and Melitta Gqada and Mireille Kamariza and Carolyn R. Bertozzi and Brian Smith and Courtney McLoud and Andrew Evan Kamholz and Wayne Bryden and Charles J. Call and Valerie Mizrahi and Robin Wood and Digby F. Warner},
  journal={bioRxiv},
  year={2019}
}
The tuberculosis (TB) pandemic demands urgent interventions such as those designed to interrupt Mycobacterium tuberculosis (Mtb) transmission, a challenge exacerbated by our poor understanding of the events enabling successful transfer of infectious bacilli between hosts. To address this problem, we developed the Respiratory Aerosol Sampling Chamber (RASC), a personal clean-room equipped with high-efficiency filtration and sampling technologies that allow biosafe capture and isolation of… 
View via Publisher
journals.plos.org
4 Citations
Background Citations
1

4 Citations

Sensitivity optimisation of tuberculosis bioaerosol sampling

Technical improvements in particle collection together with sensitive detection enable rapid quantitation of viable Mtb in bioaerosols of sputum positive TB cases and suggests the potential utility of bio aerosol measurement for rapid intervention in other airborne infectious diseases.

Cough-independent production of viable Mycobacterium tuberculosis in bioaerosol.

Bioaerosol sampling of patients with suspected pulmonary tuberculosis: a study protocol

A sensitive air sampling protocol is proposed to be used to investigate the prevalence of Mtb -containing bioaerosols in both sputum-positive and spUTum-negative TB suspects, at the same time evaluating the potential to identify unrecognized transmitters of TB.

Serial measurement of M. tuberculosis in blood from critically-ill patients with HIV-associated tuberculosis

References

SHOWING 1-10 OF 74 REFERENCES

Detection of Mycobacterium tuberculosis bacilli in bio-aerosols from untreated TB patients

Mtb was identified in bio-aerosols exhaled by the majority of untreated TB patients using the RASC, and molecular detection was more sensitive than mycobacterial culture on solid media, suggesting that further studies are required to determine whether this reflects a significant proportion of differentially detectable bacilli.

Real-Time Investigation of Tuberculosis Transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC)

Applying the RASC in a clinical setting, it is demonstrated that a combination of molecular and microbiological assays, as well as imaging by fluorescence and scanning electron microscopy, can be applied to investigate the identity, viability, and morphology of isolated aerosolized particles.

The detection of airborne Mycobacterium tuberculosis using micropore membrane air sampling and polymerase chain reaction.

The preliminary findings indicate that the technique of Micropore membrane air sampling with PCR analysis has important applications in the epidemiology and diagnosis of MTb.

Rapid detection of Mycobacterium tuberculosis in sputum with a solvatochromic trehalose probe

A 4-N,N-dimethylamino-1,8-naphthalimide–conjugated trehalose (DMN-Tre) probe that undergoes >700-fold increase in fluorescence intensity when transitioned from aqueous to hydrophobic environments, which could make it particularly useful for the rapid detection of metabolically active M. tuberculosis in resource-limited environments.

Bioaerosol production by patients with tuberculosis during normal tidal breathing: implications for transmission risk

It is provided the first evidence that intrathoracic TB increases bioaerosol production in a particle size range that could plausibly transport M. tuberculosis.

Detection and Quantification of Differentially Culturable Tubercle Bacteria in Sputum from Patients with Tuberculosis.

These observations demonstrate a novel Rpf-independent DCTB population in sputum and reveal that reduced host immunity is associated with lower prevalence of CF-responsive bacteria.

Rapid and specific labeling of single live Mycobacterium tuberculosis with a dual-targeting fluorogenic probe

A dual-targeted fluorogenic probe and microfluidic chip enables single-cell labeling and quantification of live Mycobacterium tuberculosis and shows specificity for Mtb over other bacterial species including 43 nontuberculosis mycobacteria (NTM).

Droplets, dust and guinea pigs: an historical review of tuberculosis transmission research, 1878-1940.

The literature from 1870 to 1940 was reviewed, when Mycobacterium tuberculosis was discovered and the concept of transmission emerged as a hallmark of the infectious disease.

Polymerase chain reaction used for the detection of airborne Mycobacterium tuberculosis in health care settings.

Repetitive Aerosol Exposure Promotes Cavitary Tuberculosis and Enables Screening for Targeted Inhibitors of Extensive Lung Destruction

The data show that repetitive aerosol exposure with M. tuberculosis drives greater lung damage and cavitation than a single exposure, which suggests that human lung destruction due to tuberculosis may be exacerbated in settings where individuals are repeatedly exposed.
...