Capture-ELISA based on recombinant PR3 is sensitive for PR3-ANCA testing and allows detection of PR3 and PR3-ANCA/PR3 immunecomplexes.

@article{Sun1998CaptureELISABO,
  title={Capture-ELISA based on recombinant PR3 is sensitive for PR3-ANCA testing and allows detection of PR3 and PR3-ANCA/PR3 immunecomplexes.},
  author={J Sun and David Fass and Jay A Hudson and Margaret A Viss and J{\"o}rgen Wieslander and Henry A. Homburger and Ulrich Specks},
  journal={Journal of immunological methods},
  year={1998},
  volume={211 1-2},
  pages={111-23}
}
Proteinase 3 (PR3), a constituent of azurophil granules of neutrophils (polymorphonuclear cells, PMNs), is the target antigen for most anti-neutrophil cytoplasmic antibodies (c-ANCA) in Wegener's granulomatosis (WG). We have recently developed an expression system for recombinant PR3 (rPR3) that is recognized by c-ANCA. Here, we report on the development and characterization of two monoclonal antibodies (moABs) and a rabbit polyclonal antiserum generated against this rPR3. Epitope competition… CONTINUE READING
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