Caprylic acid precipitation method for impurity reduction: an alternative to conventional chromatography for monoclonal antibody purification.

@article{Brodsky2012CaprylicAP,
  title={Caprylic acid precipitation method for impurity reduction: an alternative to conventional chromatography for monoclonal antibody purification.},
  author={Yan Brodsky and Cheng Zhang and Yinges Yigzaw and Ganesh Vedantham},
  journal={Biotechnology and bioengineering},
  year={2012},
  volume={109 10},
  pages={2589-98}
}
We report the use of caprylic acid based impurity precipitation as (1) an alternative method to polishing chromatography techniques commonly used for monoclonal antibody purification and (2) an impurity reduction step prior to harvesting the bioreactor. This impurity reduction method was tested with protein A purified antibodies and with cell culture fluid. First, the operational parameters influencing precipitation of host cell proteins and high molecular weight aggregate in protein A pools… CONTINUE READING

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