Capillary zone electrophoresis of two synthetic neuropeptides: examination of detectability and resolution as a function of peptide concentration and buffer concentration.


The capillary zone electrophoretic behavior of substance P and methionine enkephalin, when the amount of peptide was increased at a constant injection volume, was examined using a 50-microns I.D. capillary. Peak-shape distortion (asymmetry) increased when the concentration of these two peptides exceeded 1% of the buffer concentration. Increasing the buffer… (More)


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