Capillary zone electrophoresis-multiple reaction monitoring from 100 pg of RAW 264.7 cell lysate digest.

@article{Sun2013CapillaryZE,
  title={Capillary zone electrophoresis-multiple reaction monitoring from 100 pg of RAW 264.7 cell lysate digest.},
  author={Liangliang Sun and Yihan Li and Matthew M. Champion and Guijie Zhu and Roza Wojcik and Norman J. Dovichi},
  journal={The Analyst},
  year={2013},
  volume={138 11},
  pages={3181-8}
}
Capillary zone electrophoresis-multiple/single reaction monitoring (CZE-MRM/SRM), which employed an electrokinetically driven sheath-flow electrospray interface, was used for the rapid and highly sensitive detection of protein analytes in complex tryptic digests. MRM channels were developed against a commercial exponential mixture of bovine proteins. Five proteins spanning four orders of magnitude concentration range were confidently detected from only 2.5 ng of the digest mixture; the mass… CONTINUE READING