Capillary electrophoretic chiral determination of mirtazapine and its main metabolites in human urine after enzymatic hydrolysis.

@article{Santana2008CapillaryEC,
  title={Capillary electrophoretic chiral determination of mirtazapine and its main metabolites in human urine after enzymatic hydrolysis.},
  author={Fernando Jos{\'e} Malague{\~n}o de Santana and Vera Lucia Lanchote and Pierina Sueli Bonato},
  journal={Electrophoresis},
  year={2008},
  volume={29 18},
  pages={3924-32}
}
Capillary electrophoresis and liquid-phase microextraction using porous polypropylene hollow fibers were employed for the enantioselective analyses of mirtazapine and its metabolites demethylmirtazapine and 8-hydroxymirtazapine in human urine. Before the extraction, urine samples (1.0 mL) were submitted to enzymatic hydrolysis at 37 degrees C for 16 h. Then, the enzyme was precipitated with trichloroacetic acid, the pH was adjusted to 8 with 0.5 mol/L phosphate buffer solution (pH 11) and 15… CONTINUE READING