Capillary electrophoresis of double-stranded DNA fragments using a new fluorescence intercalating dye EvaGreen.

@article{Sang2006CapillaryEO,
  title={Capillary electrophoresis of double-stranded DNA fragments using a new fluorescence intercalating dye EvaGreen.},
  author={F. Tjong Kim Sang and Jicun Ren},
  journal={Journal of separation science},
  year={2006},
  volume={29 9},
  pages={1275-80}
}
EvaGreen is a new DNA intercalating dye successfully used in quantitative real-time PCR. In the present work, we firstly apply EvaGreen to the analysis of dsDNA by CE with LIF detection. Comparisons of EvaGreen dye with the commonly used dyes SYBR Green I and SYBR Gold were preformed in dsDNA analysis by CE. The linear range of dsDNA using EvaGreen was slightly wider than that using SYBR Gold and SYBR Green I, and the detection limits of dsDNA were not significantly different for the three dyes… CONTINUE READING

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