Capillary Electrophoresis

  title={Capillary Electrophoresis},
  author={Graham M. Kemp},
  journal={Biotechnology and Applied Biochemistry},
  • G. Kemp
  • Published 1 February 1998
  • Chemistry
  • Biotechnology and Applied Biochemistry
Capillary electrophoresis is a sensitive and versatile technique which has emerged over the past 10 years into the forefront of analytical methodology. Its applicability is enhanced by short separation times, facile and rapid methods of development and a requirement for very small amounts of analyte. This article reviews basic principles behind the technique and the range of options which allow the same instrument to be used for the separation and analysis of a wide range of analytes from large… 

Capillary electrophoresis of peptides

Progress in application of high performance detection schemes, namely laser‐induced fluorescence and mass spectrometry, in peptide separations by CE is presented and methodological aspects of CE separations of peptides such as selection of separation conditions, sample treatment, suppression of peptide adsorption to the capillary wall are discussed.

Capillary Electrophoresis of Oligonucleotides

  • Biology
  • 2011
Very small volumes of sample are introduced into a fused silica capillary that has an inner diameter of 10 m and is filled with a sieving matrix (gel) containing 7 M urea which denatures the oligonucleotide.

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In 1848, Louis Pasteur discovered, for the first time, a method to resolve a racemate into its two enantiomers by crystallization (Wainer and Drayer 1993). Since then, this kind of separation has

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Use of capillary electrophoresis in drug quality assessment of synthetic porcine secretin.

A comparative evaluation of the stability of test solutions was determined using CZE and HPLC over a range of pH values, andHPLC and CZE methods produced similar results at low pH.

Rapid monitoring of autolysis process of proteases by capillary electrophoresis

A protease, MCP-01, produced by a deep-sea psychrotrophic strain of Pseudoaltermonas sp. SM9913 was purified and its autolysis reaction at 20 °C–50 °C was monitored by capillary electrophoresis.

Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma by capillary electrophoresis and laser-induced fluorescence detection.

Capillary electrophoresis with laser-induced fluorescence detection allows determination of all rib oflavin vitamers far below physiological concentrations, and may become a useful tool for the assessment of riboflavin status in humans.

A Novel Micellar Electrokinetic Chromatographic Method for Separation of Metal-DDTC Complexes

The method was applied for the determination of Mo(VI) from potatoes and almond, Ni(II) from hydrogenated vegetable oil, and Co(III) from pharmaceutical preparations with RSD within 3.3% (n = 4) in terms of migration time and peak height/peak area.