Capabilities of the Falcon III detector for single-particle structure determination.

@article{Song2019CapabilitiesOT,
  title={Capabilities of the Falcon III detector for single-particle structure determination.},
  author={Boyuan Song and Julian Lenhart and Vanessa Judith Flegler and Cihan Makbul and Tim Rasmussen and Bettina B{\"o}ttcher},
  journal={Ultramicroscopy},
  year={2019},
  volume={203},
  pages={
          145-154
        }
}
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The data show that STEM imaging in general, and in particular the iDPC-STEM approach, can be applied to vitrified single-particle specimens to determine near-atomic resolution cryo-EM structures of biological macromolecules.
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TLDR
This method minimizes the amount of starting material and manipulation steps needed to go from purification to grid preparation, and could potentially be expanded to other membrane protein purification systems for its direct application in structure determination by single-particle cryo-EM.
The accuracy of protein models automatically built into cryo-EM maps with ARP/wARP.
TLDR
It is demonstrated that the local resolution is a good indicator of map interpretability, and for the majority of the test cases ARP/wARP correctly builds 90% of main-chain fragments in regions where the localresolution is 4.0 Å or better.
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A new method for accurate real-time defocus modulation in electron microscopy that will improve the configurability of the technique is presented.
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TLDR
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Elucidation of the viral disassembly switch of tobacco mosaic virus
TLDR
Two cryo‐EM structures of the helical TMV assembly at 2.0 and 1.9 Å resolution are presented and conformational details of the disassembly switch mechanism are identified: in high Ca2+/acidic pH environment, the virion is stabilized between neighboring subunits through carboxyl groups E95 and E97 in close proximity to aCa2+ binding site that is shared between two subunits.
Mechanosensitive channel gating by delipidation
TLDR
It is shown that MscS opens when it is sufficiently delipidated by incubation with the detergent dodecyl-β-maltoside or the branched detergent lauryl maltose neopentyl glycol, and that addition of detergent-solubilized lipids closes the channel even in the absence of a membrane.
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