Capabilities of the Falcon III detector for single-particle structure determination

  title={Capabilities of the Falcon III detector for single-particle structure determination},
  author={Boyuan Song and Julian Lenhart and Vanessa Judith Flegler and Cihan Makbul and Tim Rasmussen and Bettina B{\"o}ttcher},
Abstract Direct electron detectors are an essential asset for the resolution revolution in electron cryo microscopy of biological objects. The direct detectors provide two modes of data acquisition; the counting mode in which single electrons are counted, and the integrating mode in which the signal that arises from the incident electrons is integrated. While counting mode leads to far higher detective quantum efficiency at all spatial frequencies, the integrating mode enables faster data… 
Fast and accurate defocus for improved tunability of cryo-EM experiments
A method for fast and accurate defocus (FADE) modulation during movie acquisition that uses the objective lens aperture as an electrostatic pole that locally modifies the electron beam potential to enable per-frame defocus values in cryo-EM movies.
Throughput and resolution with a next-generation direct electron detector
The superior image quality given by the DE64 direct electron detector in counting mode is more important for high-resolution cryo-EM reconstructions than its superior throughput in integrating mode.
Fast Small-Scale Membrane Protein Purification and Grid Preparation for Single-Particle Electron Microscopy.
This method minimizes the amount of starting material and manipulation steps needed to go from purification to grid preparation, and could potentially be expanded to other membrane protein purification systems for its direct application in structure determination by single-particle cryo-EM.
Fast and accurate defocus modulation for improved tunability of cryo-EM experiments
A new method for accurate real-time defocus modulation in electron microscopy that will improve the configurability of the technique is presented.
CM01: a facility for cryo-electron microscopy at the European Synchrotron
Cryo-electron microscopy facility at the European Synchrotron is used by the ESRF’s international community for structural biology and aims to provide real-time information about the physical properties of Cryo-Electron MLCs.
From stars to stripes: RNA-directed shaping of plant viral protein templates-structural synthetic virology for smart biohybrid nanostructures.
  • C. Wege, Claudia Koch
  • Biology
    Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology
  • 2019
The self-assembly of viral building blocks bears exciting prospects for fabricating new types of bionanoparticles with multivalent protein shells. These enable a spatially controlled immobilization


Single particle electron cryomicroscopy: trends, issues and future perspective
Abstract There has been enormous progress during the last few years in the determination of three-dimensional biological structures by single particle electron cryomicroscopy (cryoEM), allowing maps
A Bayesian View on Cryo-EM Structure Determination
  • S. Scheres
  • Computer Science
    2012 9th IEEE International Symposium on Biomedical Imaging (ISBI)
  • 2012
The Resolution Revolution
The structure of the large subunit of the mitochondrial ribosome is reported by electron cryo-microscopy (cryo-EM), marking the beginning of a new era in molecular biology, where structures at near-atomic resolution are no longer the prerogative of x-ray crystallography or nuclear magnetic resonance (NMR) spectroscopy.