CapSeq and CIP-TAP Identify Pol II Start Sites and Reveal Capped Small RNAs as C. elegans piRNA Precursors

Abstract

Piwi-interacting (pi) RNAs are germline-expressed small RNAs linked to epigenetic programming. C. elegans piRNAs are thought to be transcribed as independent gene-like loci. To test this idea and to identify potential transcription start (TS) sites for piRNA precursors, we developed CapSeq, an efficient enzymatic method for 5' anchored RNA profiling. Using… (More)
DOI: 10.1016/j.cell.2012.11.023

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Cite this paper

@article{Gu2012CapSeqAC, title={CapSeq and CIP-TAP Identify Pol II Start Sites and Reveal Capped Small RNAs as C. elegans piRNA Precursors}, author={Weifeng Gu and Heng-Chi Lee and Daniel A. Chaves and Elaine M. Youngman and Gregory J Pazour and Darryl Conte and Craig C. Mello}, journal={Cell}, year={2012}, volume={151}, pages={1488-1500} }