Cap-dependent RNA splicing in a HeLa nuclear extract.

  title={Cap-dependent RNA splicing in a HeLa nuclear extract.},
  author={Isaac Edery and Nahum Sonenberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={82 22},
We have studied the involvement of the 5' cap structure in the splicing of precursor mRNAs in a HeLa nuclear extract. We show that precursor mRNAs are spliced efficiently only when they possess a cap structure and that preincubation of a HeLa nuclear extract rendered the splicing reaction highly sensitive to inhibition by cap analogues. This sensitization was dependent on exogenous Mg2+ but not exogenous ATP or GTP. These results demonstrate that splicing in a nuclear extract is highly… CONTINUE READING


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