Cantilever sensor for nanomechanical detection of specific protein conformations.

  title={Cantilever sensor for nanomechanical detection of specific protein conformations.},
  author={Rupa Mukhopadhyay and Vadim V. Sumbayev and Martin Lorentzen and J\orgen Kjems and Peter Andr{\'e} Andreasen and Flemming Besenbacher},
  journal={Nano letters},
  volume={5 12},
A fast, label-free, and multiplexed method based on piezoresistive cantilevers is reported for the detection of specific protein conformations at the nanoscale level. The ligand-binding domain of the human oestrogen receptor (ERalpha-LBD) is used as the experimental model system, and ERalpha-LBD with or without oestradiol (E2) is detected using the conformation-specific peptides alpha/betaI (Ser-Ser-Asn-His-Gln-Ser-Ser-Arg-Leu-Ile-Glu-Leu-Leu-Ser-Arg, which recognizes E2-bound ER) and alpha… CONTINUE READING
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