Cannabinoids Induce Cell Death and Promote P2X7 Receptor Signaling in Retinal Glial Progenitors in Culture

  title={Cannabinoids Induce Cell Death and Promote P2X7 Receptor Signaling in Retinal Glial Progenitors in Culture},
  author={H{\'e}rcules Rezende Freitas and Alinny Rosendo Isaac and Thayane Martins Silva and Geyzzara Oliveira Ferreira Diniz and Yara Santos Dabdab and Eduardo Cosendey Bockmann and Mar{\'i}lia Zaluar P. Guimar{\~a}es and Karin Costa Calaza and Fernando G. Mello and Ana L{\'u}cia Marques Ventura and Ricardo Augusto Melo Reis and Guilherme Rapozeiro França},
  journal={Molecular Neurobiology},
Development of progenitors in the embryonic retina is modulated by signaling molecules, and cannabinoid receptors are highly expressed in the early developing retina. Here, we investigated whether the CB1/CB2 receptor agonist WIN 5212-2 (WIN) modulated the proliferation, viability, and calcium responses in chick embryo retinal progenitors in culture. A decline in [3H]-thymidine incorporation was observed when cultures were incubated with 0.5–1.0 μM WIN, an effect that was mimicked by URB602 and… 
Interaction between cannabinoid and nucleotide systems as a new mechanism of signaling in retinal cell death
Evidence is emerging that cannabinoids seem to regulate neurotransmission in the retina by inhibiting the release of transmitters such as dopamine, norepinephrine, γ-aminobutyric acid and glutamate, which play an important role in retinal circuitry and in scotopic vision.
Cell Calcium Imaging as a Reliable Method to Study Neuron–Glial Circuits
Complex dynamic cellular networks have been studied in physiological and pathological processes under the light of single-cell calcium imaging (SCCI), a method that correlates functional data based
Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities
This work applied state-of-the-art label-free shotgun proteomics to compare the proteome of stem cell-derived cerebral organoids to the human fetal brain, and identified 3,073 proteins associated with different developmental stages.


Activation of Type 1 Cannabinoid Receptor (CB1R) Promotes Neurogenesis in Murine Subventricular Zone Cell Cultures
It is demonstrated that CB1R activation induces proliferation, self-renewal and neuronal differentiation from mouse neonatal SVZ cell cultures.
ATP induces the death of developing avian retinal neurons in culture via activation of P2X7 and glutamate receptors
It is suggested that ATP induces apoptosis of chick embryo retinal neurons in culture through activation of P2X7 and glutamate ionotropic receptors and involvement of a P2x7 receptor-mediated inhibition of the glial uptake of glutamate is suggested.
Interaction between Cannabinoid Type 1 and Type 2 Receptors in the Modulation of Subventricular Zone and Dentate Gyrus Neurogenesis
Findings indicate a tight interaction between CB1R and CB2R to modulate neurogenesis in the two major neurogenic niches, thus contributing to further unraveling the mechanisms behind the action of endocannabinoids in the brain.
Cannabinoid Receptor Type 1 Expression in the Developing Avian Retina: Morphological and Functional Correlation With the Dopaminergic System
A correlation between the endocannabinoid and dopaminergic systems (DSs) during the avian retina development is suggested, and activation of CB1 limits cAMP accumulation via D1 receptor activation and may influence embryological parameters during avan retina differentiation.
Non‐psychoactive CB2 cannabinoid agonists stimulate neural progenitor proliferation
It is shown that the CB2 receptor is expressed, both in vitro and in vivo, in neural progenitors from late embryonic stages to adult brain, and evidence that hippocampal progenitor proliferation is increased by administration of theCB2‐selective agonist HU‐308 is shown.
The endocannabinoid system drives neural progenitor proliferation
It is demonstrated that progenitor cells express a functional endocannabinoid system that actively regulates cell proliferation both in vitro and in vivo, and thus open novel therapeutic avenues for manipulation of NP cell fate in the adult brain.
Roles of cannabinoid receptors type 1 and 2 on the retinal function of adult mice.
The data indicated that CB2R is likely to be involved in shaping retinal responses to light and suggest that CB1 and CB2 receptors could have different roles in visual processing.