Cannabidiolic‐acid synthase, the chemotype‐determining enzyme in the fiber‐type Cannabis sativa

@article{Taura2007CannabidiolicacidST,
  title={Cannabidiolic‐acid synthase, the chemotype‐determining enzyme in the fiber‐type Cannabis sativa},
  author={Futoshi Taura and Supaart Sirikantaramas and Yoshinari Shoyama and Kazuyoshi Yoshikai and Yukihiro Shoyama and Satoshi Morimoto},
  journal={FEBS Letters},
  year={2007},
  volume={581}
}
Identification of olivetolic acid cyclase from Cannabis sativa reveals a unique catalytic route to plant polyketides
TLDR
This work shows that a type III PKS (tetraketide synthase) from cannabis trichomes requires the presence of a polyketide cyclase enzyme, olivetolic acid cyclase (OAC), which catalyzes a C2–C7 intramolecular aldol condensation with carboxylate retention to form OA.
Cannabinoids: Biosynthesis and Biotechnological Applications
TLDR
This chapter describes the history of the biosynthesis studies, in particular those focused on the biosynthetic enzymes, and recent topics linked to cannabinoid-related biotechnology.
Daurichromenic Acid-producing Oxidocyclase in the Young Leaves of Rhododendron dauricum
TLDR
Interestingly, these catalytic properties of DCA synthase are quite similar to those reported for cannabinoid synthases in the marijuana plant Cannabis sativa L.
Plant-Based Modulators of Endocannabinoid Signaling.
TLDR
The potential of plant-based secondary metabolites from Cannabis and unrelated species as ECSS modulators are highlighted and naturally occurring fatty acid amides and esters resembling anandamide and 2-arachidonoyl glycerol isolated from non- Cannabis species are valuable tools for studying ECSS function.
Recent advances in cannabinoid biochemistry and biotechnology
TLDR
This review introduces the history of cannabinoid biosynthesis studies, and focuses on the biotechnological applications of biosynthetic enzymes.
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It is concluded that CBDA is predominantly biosynthesized from cannabigerolic acid rather than cannabinerolic acid, although the kcat for the former is lower than that for the latter, and that the enzymatic cyclization does not proceed via oxygenated intermediates.
The Gene Controlling Marijuana Psychoactivity
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It is concluded that the FAD binding residue is His-114 and that the THCA synthase reaction is FAD-dependent, the first report on molecular characterization of an enzyme specific to cannabinoid biosynthesis.
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TLDR
Results showed that secretory cells of the glandular trichomes secrete not only metabolites but also biosynthetic enzyme, which indicates that THCA is biosynthesized in the storage cavity of the glands.
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In order to investigate the structure-function relationship of THCA synthase, this enzyme was overproduced in insect cells, purified and finally crystallized in 0.1 M HEPES buffer pH 7.5 containing 1.4 M sodium citrate.
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    British journal of pharmacology
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TLDR
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The cloning of a receptor for cannabinoids is reported that is not expressed in the brain but rather in macrophages in the marginal zone of spleen, which helps clarify the non-psychoactive effects of cannabinoids.
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