Viral infectivity and intracellular distribution of matrix (M) protein of canine distemper virus are affected by actin filaments
Mammalian and chick embryo adapted strains of CDV were propagated in primary ferret and dog kidney cell cultures and a continuous dog kidney cell line. CPE characterized by the formation of syncytia and/or stellate cells, together with eosinophilic cytoplasmic and intranuclear inclusions were observed. Minimal CPE, detected only in stained preparations was also observed in bovine kidney cell culture with strain 4856. However, major differences in the pattern of lesion development occurred and were related to such factors as origin of strain, passage history and host cell type. Selection of appropriate mutants was necessary to permit successful propagation of CDV strains in a given cell type. A new strain of CDV, 4856, was isolated from dog kidney cell cultures prepared from a field case of distemper. This strain was readily serially propagated and produced characteristic CPE in the four cell types studied. Measles virus produced CPE in primary ferret and dog kidney, and the continuous dog kidney cell line. The type of CPE observed was characteristic of the cell system and closely paralleled the CPE observed with CDV.