Candida arabinofermentans, a new L-arabinose fermenting yeast

  title={Candida arabinofermentans, a new L-arabinose fermenting yeast},
  author={Cletus P. Kurtzman and Bruce S. Dien},
  journal={Antonie van Leeuwenhoek},
Candida arabinofermentans (type strain NRRL YB-2248, CBS 8468), a new yeast that ferments the pentose L-arabinose, is described. The three known strains of this new species were isolated from insect frass of pine and larch trees in the U.S. Phylogenetic analysis of nucleotide sequences from the D1/D2 domain of large subunit (26S) ribosomal DNA places C. arabinofermentans among the methanol-assimilating yeasts and most closely related to Candida ovalis. Strains of the new species produce 0.7-1.9… 

Utilization and transport of l-arabinose by non-Saccharomyces yeasts

To better understand l-Arabinose transport and use in yeasts and to identify a source for efficient l-arabinose transporters, 165 non-Saccharomyces yeast strains were studied.

Characterization of Candida sp. NY7122, a novel pentose-fermenting soil yeast

The soil yeast strain NY7122 isolated from a blueberry field in Tsukuba (East Japan) could ferment both hexose and pentose (d-xylose and l-arabinose) and was closely related to Candida subhashii on the basis of the results of molecular identification.

Metabolic Engineering for Improved Fermentation of L-Arabinose.

This review presents native and engineered microorganisms that can ferment L-arabinose and Saccharomyces cerevisiae is highlighted as the most preferred engineering host for expressing a heterologous arabinose pathway for producing ethanol.

l-Arabinose metabolism in Candida arabinofermentans PYCC 5603T and Pichia guilliermondii PYCC 3012: influence of sugar and oxygen on product formation

This study demonstrates, for the first time, the oxygen dependence of metabolite and product formation in l-arabinose-assimilating yeasts.

Characterization of xylose-utilizing yeasts isolated from herbivore faeces in Thailand

A total of 39 xylose-utilizing yeast strains were isolated from herbivore faeces in Thailand based on their morphological, cultural, physiological and biochemical characteristics including the sequence analysis of the D1/D2 region of the large-subunit ribosomal DNA.

Two new anamorphic yeasts, Candida thailandica sp. nov. and Candida lignicola sp. nov., isolated from insect frass in Thailand.

Two new yeast strains of the genus Candida were isolated from insect frass collected in Khao-Yai National Park, Nakhonrachasima, Thailand and were found to represent two distinct undescribed species and were named Candida thailandica sp.

l‐Arabinose transport and catabolism in yeast

The kinetics of l‐arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties, and the first full account of the initial steps of l-arabinos catabolism in yeast including the biochemical characterization of a specific l‐Arabinose transporter is presented.

Establishment of l-arabinose fermentation in glucose/xylose co-fermenting recombinant Saccharomyces cerevisiae 424A(LNH-ST) by genetic engineering

This study reports the efficient anaerobic fermentation of l-arabinose by the derivative of 424A(LNH-ST), a genetically engineered recombinant S. cerevisiae yeast made able to efficiently ferment xylose to ethanol which was achieved by integrating multiple copies of three xylOSE-metabolizing genes.

Codon-Optimized Bacterial Genes Improve l-Arabinose Fermentation in Recombinant Saccharomyces cerevisiae

Yeast transformants expressing the codon-optimized genes showed strongly improved l-arabinose conversion rates, which make up a new starting point for the construction of more-efficient industrial l-Arabinose-fermenting yeast strains by evolutionary engineering.

Engineering of Saccharomyces cerevisiae for Efficient Anaerobic Alcoholic Fermentation of l-Arabinose

This study reports the first case of fast and efficient anaerobic alcoholic fermentation of l-arabinose by an engineered S. cerevisiae strain and efficient ethanol production from sugar mixtures containing glucose and arabinose, which is crucial for application in industrial ethanol production.



Screening for L-arabinose fermenting yeasts.

This work screened 116 different yeasts for the ability to ferment L-arabinose and found the following species able to ferment the sugar: Candida auringiensis, Candida succiphila, Ambrosiozyma monospora, and Candida sp.

Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences

Divergence in the variable D1/D2 domain of large subunit (26S) ribosomal DNA is generally sufficient to resolve individual species, resulting in the prediction that 55 currently recognized taxa are synonyms of earlier described species.

Synonomy of the yeast generaHansenula andPichia demonstrated through comparisons of deoxyribonucleic acid relatedness

It is proposed that ability to assimilate nitrate, the sole distinction betweenHansenula andPichia, is of insufficient taxonomic value for the reliable separation of either species or genera.

Strain selection, taxonomy, and genetics of xylose-fermenting yeasts

The phylogenetic relationships of the hat-shaped ascospore-forming, nitrate-assimilating Pichia species, formerly classified in the genus Hansenula Sydow et Sydow, based on the partial sequences of 18S and 26S ribosomal RNAs (Saccharomycetaceae): the proposals of three new genera, Ogataea, Kuraishia

The twenty-seven strains of the hat-shaped ascospore-forming, nitrate-assimilating species, formerly classified in the genus Hansenula, of the genus Pichia were examined for their 18S and 26S rRNA partial base sequencings, and seven groupings are discussed phylogenetically and taxonomically.

Phylogenetic relationships among species of Saccharomyces, Schizosaccharomyces, Debaryomyces and Schwanniomyces determined from partial ribosomal RNA sequences

Schizosac charomyces proved to be somewhat more divergent than Saccharomyces and Debaryomyces, but species differences appear insufficient for dividing the genus, and some of the factors influencing estimates of phylogenetic distances from rRNA sequences are discussed.

Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA gene

Of the 204 described species examined, 21 appeared to be synonyms of previously described organisms, and the relationships among the species are presented.


Phylogenetic analyses showed that Stephanoascus is distinct from Zygoascus and that Blastobotrys, Sympodiomyces, and Arxula represent anamorphs of the Stephano Damascus clade.

A phylogenetic analysis of the genus Saccharomyces based on 18S rRNA gene sequences: description of Saccharomyces kunashirensis sp. nov. and Saccharomyces martiniae sp. nov.

A phylogenetic investigation of the ascomycetous yeast genus Saccharomyces was performed by using 18S rRNA gene sequence analysis, showing that the genus is phylogenetically very heterogeneous.

Methods for the isolation, maintenance and identification of yeasts