Candida albicans Gene Deletion with a Transient CRISPR-Cas9 System

  title={Candida albicans Gene Deletion with a Transient CRISPR-Cas9 System},
  author={Kyunghun Min and Yuichi Ichikawa and Carol A Woolford and Aaron P. Mitchell},
Clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated gene 9 (CRISPR-Cas9) systems are used for a wide array of genome-editing applications in organisms ranging from fungi to plants and animals. Recently, a CRISPR-Cas9 system has been developed for the diploid fungal pathogen Candida albicans; the system accelerates genetic manipulation dramatically [V. K. Vyas, M. I. Barrasa, and G. R. Fink, Sci Adv 1(3):e1500248, 2015,… CONTINUE READING
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