Cancer gene therapy: A bystander effect using the gap junctional pathway

  title={Cancer gene therapy: A bystander effect using the gap junctional pathway},
  author={John D. Pitts},
  journal={Molecular Carcinogenesis},
  • J. Pitts
  • Published 1 November 1994
  • Biology
  • Molecular Carcinogenesis

Gemcitabine intercellular diffusion mediated by gap junctions: new implications for cancer therapy

It is suggested that gemcitabine treatment could be more efficient for treating solid tumors that display gap junctions, and the presence of these cellular channels could be used to predict the responsiveness to this nucleoside analogue therapy.

Bystander effect in the adenovirus-mediated wild-type p53 gene therapy model of human squamous cell carcinoma of the head and neck.

The bystander effect in the adenovirus-mediated wild-type p53 gene therapy model of squamous cell carcinoma of the head and neck, when present, requires intercellular contact and possible mechanisms of the observed in vitro bystanders effect are discussed.

Solute exchange through gap junctions lessens the adverse effects of inactivating mutations in metabolite-handling genes

This work shows that diffusive coupling was able to rescue the functional defect associated with the inactivation of metabolite-handling genes, and can explain why certain loss-of-function mutations in genes do not influence the growth of human cancers.

Radiation-Induced Bystander Effect can be Transmitted Through Exosomes Using miRNAs as Effector Molecules

After investigating the field of RIBE, it is important to understand the mechanisms and consequences of biological effects as well as the role of exosomes and exosomal miRNAs therein, from different sources and under different circumstances, respectively.

Connexins in cancer: bridging the gap to the clinic

The current understanding of the role of connexins and gap junctions in cancer is reviewed, with particular focus on the recent progress made in determining their prognostic and therapeutic potential.

University of Groningen Ovarian cancer gene therapy

This review provides an overview of existing gene therapy strategies for ovarian carcinoma and attempts to develop strategies for their targeted delivery to ovarian carcinomas to increase the therapeutic index of gene therapy.

Hyaluronan-conjugated liposomes encapsulating gemcitabine for breast cancer stem cells

It is demonstrated that HA-conjugated liposomes encapsulating GEM show promise for the therapy of breast cancer in vitro and in a xenograft model by targeting the BCSCs.

Gap junctions and cancer: communicating for 50 years

This Timeline article highlights the milestones connecting gap junctions to cancer, and underscores important unanswered questions, controversies and therapeutic opportunities in the field.



Hypoxanthine-guanine phosphoribosyltransferase deficiency: chemical agents selective for mutant or normal cultured fibroblasts in mixed and heterozygote cultures.

Cultured fibroblasts established from skin biopsies from patients with the Lesch-Nyhan syndrome are deficient in hypoxanthine-guanine phosphoribosyl-transferase (EC, and two-way selection has been achieved by the use of 6-thioguanine and azaserine, which selects for the deficient mutant and for the normal allele.

Retroviral-mediated gene therapy for the treatment of hepatocellular carcinoma: an innovative approach for cancer therapy.

Developing the VDEPT approach for the treatment of hepatocellular carcinoma by utilizing retroviral vectors that were constructed containing a chimeric varicella-zoster virus thymidine kinase gene that is transcriptionally regulated by either the hepatoma-associated alpha-fetoprotein or liver-associated albumin transcriptional regulatory sequences.

Transformation-dependent expression of interleukin genes delivered by a recombinant parvovirus

The recombinant viruses were able to transfer fully functional IL-2 and IL-4 genes to permissive target cells and retained the oncotropic host range properties of the parental virus.

Junctional intercellular communication and the control of growth.

Patterns of junctional communication in skin.

The patterns of junctional communication in whole skin have been studied by iontophoretic injection of the fluorescent dye Lucifer Yellow CH into excised strips of tissue from newborn (less than

Cell-to-cell communication within intact human skin.

Cell coupling was found to be more extensive in epidermal ridges than in suprapapillary plates and was less extensive after injection of the basal layer of the epidermis than after that of the suprabasal layers.

Involvement of gap junctions in tumorigenesis: transfection of tumor cells with connexin 32 cDNA retards growth in vivo.

It is demonstrated that growth rate of these tumor cells in situ is negatively correlated with strength of intercellular communication.