Can yeast glycolysis be understood in terms of in vitro kinetics of the constituent enzymes? Testing biochemistry.

@article{Teusink2000CanYG,
  title={Can yeast glycolysis be understood in terms of in vitro kinetics of the constituent enzymes? Testing biochemistry.},
  author={Bas Teusink and Jutta Passarge and C A Reijenga and E Esgalhado and Coen C van der Weijden and M. Schepper and Michael C. Walsh and Barbara M. Bakker and Koen van Dam and Hans V. Westerhoff and Jacky L. Snoep},
  journal={European journal of biochemistry},
  year={2000},
  volume={267 17},
  pages={5313-29}
}
This paper examines whether the in vivo behavior of yeast glycolysis can be understood in terms of the in vitro kinetic properties of the constituent enzymes. In nongrowing, anaerobic, compressed Saccharomyces cerevisiae the values of the kinetic parameters of most glycolytic enzymes were determined. For the other enzymes appropriate literature values were collected. By inserting these values into a kinetic model for glycolysis, fluxes and metabolites were calculated. Under the same conditions… CONTINUE READING
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