Can hammerhead ribozymes be efficient tools to inactivate gene function?

@article{Bertrand1994CanHR,
  title={Can hammerhead ribozymes be efficient tools to inactivate gene function?},
  author={Edouard Bertrand and R. Pictet and Thierry Grange},
  journal={Nucleic acids research},
  year={1994},
  volume={22 3},
  pages={293-300}
}
In order to improve hammerhead ribozyme efficiency and specificity, we have analyzed, both in vitro and in vivo, the activity of a series of ribozyme/substrate combinations that have the same target sequence but differ in the length of the ribozyme/substrate duplex or in their structure, i.e., the total length of the RNA. In vitro, we have found that optimal kcat/Km (at 37 degrees C) is obtained when the ribozyme/substrate duplex has a length of 12 bases, which according to the base composition… CONTINUE READING