Can hTNF-α be successfully produced and secreted in filamentous fungus Aspergillus niger?


A gene-fusion expression strategy was applied for the heterologous expression of hTNF-α in A. niger AB1.13. The TNF-α gene was fused with the A. niger glucoamylase GII form as a carrier-gene, behind its transcription control and secretion signal. The protein was expressed in the cells in the form of a glucoamylase-fusion protein, but was not present in the… (More)
DOI: 10.1007/s004240000100

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