Campbell-like integration of heterologous plasmid DNA into the chromosome of Lactococcus lactis subsp. lactis.

@article{Leenhouts1989CampbelllikeIO,
  title={Campbell-like integration of heterologous plasmid DNA into the chromosome of Lactococcus lactis subsp. lactis.},
  author={Kees J Leenhouts and Jan Kok and Gerard A. Venema},
  journal={Applied and environmental microbiology},
  year={1989},
  volume={55 2},
  pages={394-400}
}
Integrable vectors were constructed based on the plasmid pHV60, which is essentially a pBR322 replicon carrying a chloramphenicol resistance marker, by inserting 1.3-kilobase chromosomal fragments of Lactococcus lactis subsp. lactis MG1363 into this plasmid. Three constructs as well as pHV60 were electroporated to strain MG1363. Transformants were obtained with all constructs, and also with pHV60 (albeit with low frequency). By using Southern hybridizations, it appeared that pHV60 showed… CONTINUE READING

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