• Corpus ID: 59604436

Cameraless High-throughput 3D Imaging Flow Cytometry

  title={Cameraless High-throughput 3D Imaging Flow Cytometry},
  author={Yuanyuan Han and Rui Tang and Yi Gu and Alex Ce Zhang and Wei Cai and Violet Castor and Sung Hwan Cho and William A. Alaynick and Yu-Hwa Lo},
  journal={arXiv: Quantitative Methods},
Increasing demand for understanding the vast heterogeneity of cellular phenotypes has driven the development of imaging flow cytometry (IFC), that combines features of flow cytometry with fluorescence and bright field microscopy. IFC combines the throughput and statistical advantage of flow cytometry with the ability to discretely measure events based on a real or computational image, as well as conventional flow cytometry metrics. A limitation of existing IFC systems is that, regardless of… 

Figures from this paper


Imaging Cells in Flow Cytometer Using Spatial-Temporal Transformation
A method of spatial-temporal transformation is invented to provide flow cytometers with cell imaging capabilities and instead of CCDs or any megapixel cameras found in any imaging systems, high quality image of fast moving cells is obtained using PMT detectors, thus obtaining high throughput in manners fully compatible with existing cytometers.
High-throughput single-microparticle imaging flow analyzer
An automated flow-through single-particle optical microscope is presented that performs sensitive blur-free image acquisition and nonstop real-time image-recording and classification of microparticles during high-speed flow and demonstrates high-throughput image-based screening of budding yeast and rare breast cancer cells in blood.
Ghost cytometry
Seeing ghosts In fluorescence-activated cell sorting, characteristic target features are labeled with a specific fluorophore, and cells displaying different fluorophores are sorted. Ota et al.
Optical sectioning microscopy
The core concepts of confocal microscopes and important variables that adversely affect confocal images are described and computational optical sectioning techniques that can perform Optical sectioning without a confocal microscope are discussed.
FISH-quant: automatic counting of transcripts in 3D FISH images
FISH-QUANT is developed to close the gap in single-molecule RNA fluorescence in-situ hybridization that cannot reliably quantify the dense mRNA aggregates at transcription sites (TS) in three dimensions (3D), particularly of highly transcribing genes.
Review: imaging technologies for flow cytometry.
Current imaging flow cytometry technologies are remarkably revolutionizing single-cell analysis and their challenges are described.
Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging
Results were consistent with Raman spectral analysis, indicating that the proposed PSI/AFM based refractive index system is likely to become a useful tool for intracellular biochemical components analysis measurement, and this will facilitate its application for revealing cell structure and pathological state from a new perspective.
3D-structured illumination microscopy reveals clustered DNA double-strand break formation in widespread γH2AX foci after high LET heavy-ion particle radiation
This work identified the formation of 3D widespread γH2AX foci after high LET carbon-ion irradiation in G2-phase cells and suggests that high LET heavy-ion particles induce clustered DSB formation on a scale of approximately 1 μm3.