Calving sex ratio as related to the predicted Y-chromosome-bearing spermatozoa ratio in bull ejaculates.

@article{Chandler2007CalvingSR,
  title={Calving sex ratio as related to the predicted Y-chromosome-bearing spermatozoa ratio in bull ejaculates.},
  author={John E Chandler and Tara M Taylor and Anita L Canal and Richard Kent Cooper and E. B. Moser and Michael E McCormick and Scott T. Willard and Herb E Rycroft and Glen R Gilbert},
  journal={Theriogenology},
  year={2007},
  volume={67 3},
  pages={563-71}
}
The first objective was to correlate calving sex-ratio data from semen lots with the semen sex ratio obtained by two duplex polymerase chain reaction (PCR)/gel electrophoresis techniques. The two techniques involved different starting DNA amounts, PCR conditions, agarose gel concentrations, sample placement on the gels, lane size, number of lanes per gel, and duration of electrophoresis. The second objective was to sequence the duplex PCR products to verify their match to genes and chromosomes… CONTINUE READING

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