Calpain mutants with increased Ca2+ sensitivity and implications for the role of the C(2)-like domain.

@article{Hosfield2001CalpainMW,
  title={Calpain mutants with increased Ca2+ sensitivity and implications for the role of the C(2)-like domain.},
  author={Christopher M. Hosfield and Tudor Moldoveanu and Peter L Davies and John S. Elce and Zhonghua Jia},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 10},
  pages={7404-7}
}
The ubiquitous calpain isoforms (mu- and m-calpain) are Ca(2+)-dependent cysteine proteases that require surprisingly high Ca(2+) concentrations for activation in vitro ( approximately 50 and approximately 300 microm, respectively). The molecular basis of such a high requirement for Ca(2+) in vitro is not known. In this study, we substantially reduced the concentration of Ca(2+) required for the activation of m-calpain in vitro through the specific disruption of interdomain interactions by… CONTINUE READING

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