Calorimetric study of the heat and cold denaturation of beta-lactoglobulin.

  title={Calorimetric study of the heat and cold denaturation of beta-lactoglobulin.},
  author={Y V Griko and Peter L. Privalov},
  volume={31 37},
Temperature-induced changes of the states of beta-lactoglobulin have been studied calorimetrically. In the presence of a high concentration of urea this protein shows not only heat but also cold denaturation. Its heat denaturation is approximated very closely by a two-state transition, while the cold denaturation deviates considerably from the two-state transition and this deviation increases as the temperature decreases. The heat effect of cold denaturation is opposite in sign to that of heat… CONTINUE READING