Caloric restriction reduces fiber loss and mitochondrial abnormalities in aged rat muscle

  title={Caloric restriction reduces fiber loss and mitochondrial abnormalities in aged rat muscle},
  author={Lauren E. Aspnes and Connie M Lee and R. H. Weindruch and Susan S. Chung and Ellen B. Roecker and Judd M. Aiken},
  journal={The FASEB Journal},
  pages={573 - 581}
The influence of caloric restriction (CR) initiated at 17 months of age was investigated on selected age‐associated measures in skeletal muscle. Tissue from young (3–4 months) ad libitum‐fed, old (30–32 months) restricted (35% and 50% CR, designated CR35 and CR50, respectively), and old ad libitum‐fed rats (29 months) was studied. CR preserved fiber number and fiber type composition in the vastus lateralis muscle of the CR50 rats. In the old rats from all groups, individual fibers were found… 

Early‐onset calorie restriction conserves fiber number in aging rat skeletal muscle

Calorie restriction did not prevent muscle mass loss with age; however, it significantly reduced Muscle mass loss between 21 and 36 months of age compared with age‐ matched AL cohorts.

Synergistic effects of caloric restriction with maintained protein intake on skeletal muscle performance in 21‐month‐old rats: a mitochondria‐mediated pathway

The synergistic effects of CR with maintained protein intake may help to limit the progression of sarcopenia by optimizing the turnover rates and functions of major proteins in skeletal muscle.

Calorie restriction limits the generation but not the progression of mitochondrial abnormalities in aging skeletal muscle

Calorie restriction affects the onset but not the progression of electron transport system abnormalities, thereby, limiting a process that ultimately results in fiber breakage and fiber loss.

Effectiveness of caloric restriction in preventing age-related changes in rat skeletal muscle.

Results show that calorie restriction preserves the mechanical properties of aging hind-limb skeletal muscle and maintains the level of DHPR and RyR1 in aged F344BNF1 rats fed ad libitum.

No decline in skeletal muscle oxidative capacity with aging in long-term calorically restricted rats: effects are independent of mitochondrial DNA integrity.

There was a slower decline in PGC-1alpha gene expression in CR versus AL animals, suggesting a better maintenance of mitochondrial biogenesis with aging in CR animals, independent of mitochondrial DNA damage between groups.

Long‐term caloric restriction abrogates the age‐related decline in skeletal muscle aerobic function

Interestingly, although most markers of oxidative capacity began at a lower point in young adult CR animals, CR rats exhibited a higher in situ activity of complex IV at VO2max, which allows the young adultCR animals to exhibit normal aerobic capacity despite the lower oxidative enzyme activities.

Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle

This study is the first to quantify morphologically and localize the agedependent accrual of oxidative damage in mammalian skeletal muscle and to demonstrate that oxidativeDamage in primates is lowered by CR.

Skeletal muscle aging in F344BN F1-hybrid rats: I. Mitochondrial dysfunction contributes to the age-associated reduction in VO2max.

The hypothesis that a reduced oxidative capacity, due in part to age-related mitochondrial dysfunction, contributes to the decline in aerobic performance in aging skeletal muscles is supported.



Dietary restriction attenuates age-related increases in rat skeletal muscle antioxidant enzyme activities.

The influences of age and DR on the antioxidant enzymes catalase, glutathione peroxidase, and superoxide dismutase in skeletal muscle from 11, 26, and 34-mo-old rats fed either ad libitum or subjected to a 30% DR from 14 weeks of age are investigated.

Muscle atrophy and hypoplasia with aging: impact of training and food restriction.

Fiber number for soleus and EDL muscles of combined groups declined with age by 5.6 and 4.2%, respectively, which can account at most for approximately 25% of the observed skeletal muscle atrophy.

Influence of fat intake and caloric restriction on bone in aging male rats.

Higher dietary fat content did not affect BW in CR animals, but did result in lower distal femur BMC, which was entirely accounted for by reduction in BW.

Modification of mitochondrial respiration by aging and dietary restriction

Influence of nutrition and aging on the composition and function of rat skeletal muscle.

The results suggest that the fibers present in the muscles of aged rats are functionally intact and are unaffected by long-term dietary restriction, suggesting that age-related deficiencies of motor function are probably related to other factors, such as those associated with neuromuscular transmission or propagation of nerve impulses.

Influences of age and dietary restriction on gastrocnemius electron transport system activities in mice.

Alterations in the mitochondrial electron transport system (ETS) may contribute to aging as well as decreases in skeletal muscle function through alterations with aging in mitochondrial ETS capacities.

Effect of age and caloric restriction on DNA oxidative damage in different tissues of C57BL/6 mice

Alteration of antioxidant enzymes with aging in rat skeletal muscle and liver.

It is concluded that aging is accompanied with an elevation of antioxidant enzyme activities and lipid peroxidation in skeletal muscle probably due to the increased oxygen free radical production and reaction.

Skeletal muscle atrophy in old rats: Differential changes in the three fiber types