Calmodulin concentrates at regions of cell growth in Saccharomyces cerevisiae

@article{Brockerhoff1992CalmodulinCA,
  title={Calmodulin concentrates at regions of cell growth in Saccharomyces cerevisiae},
  author={Susan E Brockerhoff and Trisha N. Davis},
  journal={The Journal of Cell Biology},
  year={1992},
  volume={118},
  pages={619 - 629}
}
Calmodulin was localized in Saccharomyces cerevisiae by indirect immunofluorescence using affinity-purified polyclonal antibodies. Calmodulin displays an asymmetric distribution that changes during the cell cycle. In unbudded cells, calmodulin concentrates at the presumptive site of bud formation approximately 10 min before bud emergence. In small budded cells, calmodulin accumulates throughout the bud. As the bud grows, calmodulin concentrates at the tip, then disperses, and finally… CONTINUE READING
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This result indicated that yeast calmodulin, free of any other yeast protein, was sufficient to compete away the immunofluorescent signal seen in yeast

  • Davis
  • 1992
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4 Excerpts

Strocua'al analysis of wild-type and mutant yeast calmodulins by limited proteolysis and electrospray ionization mass spectrometry

  • S. E. Brockerhoff, T. N. Davis.
  • Prof. Sci. 1:504-516.
  • 1992

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