• Corpus ID: 56033893

Callus culture of Aspidosperma ramiflorum Muell . Arg . : growth and alkaloid production

@inproceedings{Oliveira2004CallusCO,
  title={Callus culture of Aspidosperma ramiflorum Muell . Arg . : growth and alkaloid production},
  author={Arildo Jos{\'e} Braz de Oliveira and Luzia Koike and Francisco de A.M. Reis and Simone Liliane Kirszenzaft Shepherd},
  year={2004}
}
Callus culture of Aspidosperma ramiflorum was established in Murashige and Skoog medium. Callus were supplemented with 1 mg.L 2,4-dichlorophenoxyacetic acid, 1.5 mg.L benzylaminopurine, 30 g L sucrose and 10 g L agar. Cultures accumulated the same major alkaloids, ramiflorine A and ramiflorine B, present in the stem bark of the parent plant. The alkaloid contents of 10-methoxy-geissoschizol, ramiflorine A and ramiflorine B, of bark and callus cultures were quantitatively compared by HPLC. 

Figures and Tables from this paper

Improved alkaloid content in callus culture of Catharanthus roseus
TLDR
It was found that half strength MS basal medium supplemented with 2, 4-D and BA and 6% sucrose was best for biomass production of leaf callus and enhancement of alkaloid accumulation in C. roseus.
Tropane Alkaloid Production via New Promising Atropa belladonna L. Lines by In Vivo and In Vitro
Atropa belladonna L. seeds of the three promising new lines (M-11-1, M-11-2 and M-15-1) were used to produce alkaloids especially atropine via in vivo and in vitro. Highly significant effect on lines
CALLUS INDUCTION AND IN VITRO MULTIPLICATION OF BOERHAAVIA DIFFUSA - MILESTONE MEDICINAL PLANT OF JHARKHAND
TLDR
An attempt was taken to improve the yield and quality of Boerhavia diffusa, by callogenesis from leaf and nodal explant using different combination of Auxin and Cytokinin with or without addatives (citric acid, ascorbic acid and coconut water).
Reserpine Accumulation in NaCl Treated Calli of Rauvolfia tetraphylla L.
Rauvolfia tetraphylla L. is an endangered plant, known for its medicinal properties. It contains various indole group alkaloids with reserpine most prominent among them. Reserpine is a hypotensive
A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures.
TLDR
The present review highlights the nature, applications, perspective and scale up methods for the production of valuable secondary metabolites in vitro.
Anti-leishmanial activity of alkaloidal extract from Aspidosperma ramiflorum.
TLDR
In vitro results against L. (L.) amazonensis show the effectiveness of an alkaloid extract of Aspidosperma ramiflorum Muell against protozoa of the genus Leishmania, and new studies should be made to find the compounds with anti-leishmanial activity.
Secondary Metabolites of Plants and their Role: Overview
TLDR
A continuation and intensification of efforts in this field is expected to lead to successful biotechnological production of specific, valuable and as yet unknown plant chemicals.
Atividade biológica e alcalóides indólicos do gênero Aspidosperma (Apocynaceae): uma revisão
TLDR
A review of the main reports involving chemical and biological studies on the genus Aspidosperma aimed at improving the knowledge on the importance of indole alkaloids in the genus and their relationship with attributed or tested pharmacological activities.
Bioactive Natural Products from Plants and Biotechnological Approaches for their Production
TLDR
New and powerful tools in functional genomics can be used in combination with metabolomics to elucidate biosynthetic pathways of natural products, and genetic fingerprinting could be a powerful tool in the field of medicinal plants to be used for correct germplasm identification.
EXPLORING THE ANTI-LEISHMANIAL ACTIVITY OF OF PHOENIX DACTYLIFERA (DATE PALM)
TLDR
*N.Al-wesali, Sahibzada Tasleem Rasool, Farag Ali Saleh Ibrahim, Faragh Ali SaleH Ibrahim, Sree Harsha, Kumar GS, Mohammed S.
...
...

References

SHOWING 1-7 OF 7 REFERENCES
PLANT CELL BIOTECHNOLOGY FOR THE PRODUCTION OF ALKALOIDS: PRESENT STATUS AND PROSPECTS'
TLDR
A review of the production of the economically important alkaloids in plant cell cultures shows that presently only berberine and sanguinarine are being produced, and it seems that compartmentation also plays a role in the regulation of alkaloid biosynthesis.
Estudo de seis especies do genero aspidosperma utilizando GC, GC/MS e HPLC : analise qualitativa e quantitativa. Teste bioautografico; cultura de tecidos e celulas vegetais e rota de preparação dos compostos dimericos ramiflorina A e ramiflorina B
O desenvolvimento de um metodo de analise rapido e economico de plantas contendo alcaloides indolicos utilizando CG, CG/EM e CLAE foi a principal proposicao desta tese. Nosso grupo de pesquisa tem
Árvores brasileiras. Nova Odessa: Plantarum,1992
  • 1992