• Corpus ID: 56033893

Callus culture of Aspidosperma ramiflorum Muell . Arg . : growth and alkaloid production

@inproceedings{Oliveira2004CallusCO,
  title={Callus culture of Aspidosperma ramiflorum Muell . Arg . : growth and alkaloid production},
  author={Arildo Jos{\'e} Braz de Oliveira and Luzia Koike and Francisco de A.M. Reis and Simone Liliane Kirszenzaft Shepherd},
  year={2004}
}
Callus culture of Aspidosperma ramiflorum was established in Murashige and Skoog medium. Callus were supplemented with 1 mg.L 2,4-dichlorophenoxyacetic acid, 1.5 mg.L benzylaminopurine, 30 g L sucrose and 10 g L agar. Cultures accumulated the same major alkaloids, ramiflorine A and ramiflorine B, present in the stem bark of the parent plant. The alkaloid contents of 10-methoxy-geissoschizol, ramiflorine A and ramiflorine B, of bark and callus cultures were quantitatively compared by HPLC. 
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