Calibration procedures for the quantitative determination of membrane potential in human cells using anionic dyes.

@article{Klapperstueck2013CalibrationPF,
  title={Calibration procedures for the quantitative determination of membrane potential in human cells using anionic dyes.},
  author={Thomas Klapperstueck and Dagobert Glanz and Stefan Hanitsch and Manuela Klapperstueck and Fritz Markwardt and Johannes Wohlrab},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  year={2013},
  volume={83 7},
  pages={612-26}
}
Quantitative determinations of the cell membrane potential of lymphocytes (Wilson et al., J Cell Physiol 1985;125:72-81) and thymocytes (Krasznai et al., J Photochem Photobiol B 1995;28:93-99) using the anionic dye DiBAC4 (3) proved that dye depletion in the extracellular medium as a result of cellular uptake can be negligible over a wide range of cell densities. In contrast, most flow cytometric studies have not verified this condition but rather assumed it from the start. Consequently, the… CONTINUE READING