Calibrated brightfield-based imaging for measuring intracellular protein concentration.

Abstract

Intracellular protein concentration is an essential cell characteristic, which manifests itself through the refractive index. The latter can be measured from two or more mutually defocused brightfield images analyzed using the TIE (transport-of-intensity equation). In practice, however, TIE does not always achieve quantitatively accurate results on… (More)
DOI: 10.1002/cyto.a.23145

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