Calf spleen NAD+ glycohydrolase: solubilization, purification, and properties of the intact form of the enzyme.


NAD+ glycohydrolase was solubilized from calf spleen microsomes with emulphogene, a nonionic detergent, and purified to apparent homogeneity by ion-exchange chromatographies and by affinity chromatography on Affi-Gel blue gel. In contrast to the hydrosoluble form of the enzyme, which can be obtained by a proteolytic treatment of the microsomes with steapsin… (More)


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