Calelectrin, a Calcium‐Dependent Membrane‐Binding Protein Associated with Secretory Granules in Torpedo Cholinergic Electromotor Nerve Endings and Rat Adrenal Medulla

@article{Walker1983CalelectrinAC,
  title={Calelectrin, a Calcium‐Dependent Membrane‐Binding Protein Associated with Secretory Granules in Torpedo Cholinergic Electromotor Nerve Endings and Rat Adrenal Medulla},
  author={J. H. Walker and Jost Obrocki and Thomas C. S{\"u}dhof},
  journal={Journal of Neurochemistry},
  year={1983},
  volume={41}
}
Abstract: Calelectrin, a calcium‐dependent membrane‐binding protein of subunit molecular weight 32,000 has been isolated from the electric organ of Torpedo, and shown to occur in cholinergic neurones and in bovine adrenal medulla. In this study a monospecific antiserum against the Torpedo protein has been used to study the localization of calelectrin in the rat adrenal gland. The cortex was not stained, whereas in the medulla the cytoplasm of the chromaffin cells was stained in a particulate… 
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Characterization of Calelectrin, a Ca2+‐Binding Protein Isolated from the Electric Organ of Torpedo marmorata
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High-level expression of the 32.5-kilodalton calelectrin in ductal epithelia as revealed by immunocytochemistry.
TLDR
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References

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Isolation from Cholinergic Synapses of a Protein That Binds to Membranes in a Calcium‐Dependent Manner
  • J. Walker
  • Biology, Chemistry
    Journal of neurochemistry
  • 1982
TLDR
A protein that binds to membranes in a calcium‐dependent manner between calcium concentrations of 10−5 and 10−6M has been isolated in large amounts from the entirely cholinergic electric organ of Torpedo marmorata and was localised in the synaptic region of the electric organ by means of immunofluorescence.
Calcium‐Dependent Binding of Cytosolic Proteins by Chromaffin Granules from Adrenal Medulla
TLDR
Purified chromaffin granules from bovine adrenal medulla bound a small group of medullary cell cytosol proteins at micromolar levels of Ca2+ and physiological levels of K+, Mg2+, and Mg‐ATP, which did not correspond with any previously reported cytosolic components of Chromaffin cells.
Calelectrin self‐aggregates and promotes membrane aggregation in the presence of calcium.
TLDR
The Ca2+‐induced self‐association of calelectrin and its aggregation enhancing effect may be of great importance to the structural organization of neural and secretory cells and the mechanism of exocytosis.
SOLUBLE AND MEMBRANE LECTIN‐BINDING GLYCOPROTEINS OF THE CHROMAFFIN GRANULE
Abstract— Fluorescein isothiocyanate‐labelled lectins were used to identify lectin‐binding glycoproteins of the chromaffin granule after electrophoresis of the membrane and soluble granule proteins
Chemical composition of cholinergic synaptic vesicles from Torpedo marmorata based on improved purification.
TLDR
Cholinergic synaptic vesicles from the electric organ of Torpedo marmorata have been purified to a constant composition and a higher transmitter content than previously reported, and characterisation by two-dimensional gel electrophoresis suggested that it is an actin-like polypeptide.
Mechanochemical properties of brain clathrin: interactions with actin and alpha-actinin and polymerization into basketlike structures or filaments.
TLDR
Because of clathrin's biochemical and biophysical properties, its interaction with contractile proteins, and its presence in the membrane of vesicles of various cell types, it is classified in the group of mechanochemical proteins.
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TLDR
Fodrin is a specific component of the cortical cytoplasm of many cells and the possibilities are considered: (a) that fodrin may be indirectly attached to the plasma membrane via cortical actin filaments; (b) that F-actin could serve to link other proteins and organelles to a submembrane force-generating system.
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