Calculation of membrane potential in synaptosomes with use of a lipophilic cation (tetraphenylphosphonium).

@article{Aiuchi1989CalculationOM,
  title={Calculation of membrane potential in synaptosomes with use of a lipophilic cation (tetraphenylphosphonium).},
  author={Toshihiro Aiuchi and Masayuki Matsunaga and Ken-ichi Nakaya and Yoshikazu Nakamura},
  journal={Chemical & pharmaceutical bulletin},
  year={1989},
  volume={37 12},
  pages={3333-7}
}
To estimate membrane potential in synaptosomes with the use of tetraphenylphosphonium (TPP+), an equation relating the amount of TPP+ accumulated in synaptosomes with membrane potential was derived from the following two assumptions. (1) TPP+ molecules were distributed into plasma membranes, mitochondria and cytosol of synaptosomes. (2) TPP+ achieves a Nernst equilibrium across both the synaptosomal and inner mitochondrial membranes. We propose three methods for calculation of membrane… CONTINUE READING