Calculating molar absorptivities for quinones: application to the measurement of tyrosinase activity.

@article{Muoz2006CalculatingMA,
  title={Calculating molar absorptivities for quinones: application to the measurement of tyrosinase activity.},
  author={Jos{\'e} Luis Pedraz Mu{\~n}oz and Francisco Garc{\'i}a-Molina and R. Var{\'o}n and Jos{\'e} Neptuno Rodr{\'i}guez-L{\'o}pez and Francisco Garc{\'i}a-C{\'a}novas and Jos{\'e} I Tudela},
  journal={Analytical biochemistry},
  year={2006},
  volume={351 1},
  pages={128-38}
}
The molar absorptivities of the quinones produced from different o-diphenols, triphenols, and flavonoids were calculated by generating the respective quinones through oxidation with an excess of periodate. Oxidation of these substrates by this reagent was analogous to oxidation by tyrosinase with molecular oxygen, although the procedure showed several advantages over the enzymatic method in that oxidation took place almost immediately and quinone stability was favored because no substrate… CONTINUE READING

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