Calcofluor disrupts the midgut defense system in insects.

  title={Calcofluor disrupts the midgut defense system in insects.},
  author={Ping Wang and Robert R. Granados},
  journal={Insect biochemistry and molecular biology},
  volume={30 2},
The insect midgut is generally lined with a unique protective chitin/protein structure, the peritrophic membrane (PM). We demonstrated that in Trichoplusia ni larvae, the majority of PM proteins were assembled with chitin as a consequence of their chitin binding properties. These proteins could be dissociated from the PM in vitro by Calcofluor, a well-known chemical with chitin binding properties. The chitin binding characteristics of PM proteins were confirmed by their high affinity binding in… CONTINUE READING